Proteome Research

Tosifusa Toda, Ph.D.
（Concurrent: TMIG Proteomics Collaboration Center, Head）


Machiko Iwamoto, Ph.D., Hiraku Morisawa, M.S.

proteome, proteomics, oxidative stress, posttranslational modification, cellular aging, , brain aging, neurodegenerative disorders, disease biomarkers


2. Proteome research on the mechanisms of oxidative stress-induced cellular damages.

3. Proteomic survey for protein disease markers.

4. Development of advanced LIMS for proteome research.


 

 　Functional decline observed in aged animal tissues is thought to be a result of cellular aging. Though the principal process of somatic cell aging basically depends on genomic instructions, phenotypes of aged cells are expressed in a given internal environment of each cell type that is constructed with translated and posttranslationally modified proteins. Therefore, research on age-dependent protein alterations in each cell type is very important for clarifying mechanisms of aging. The novel term "proteome" is a compound of "protein" and "-ome," which means constitutive whole proteins including posttranslationally modified products in a given cell type. Proteomics is a strategy for analyzing proteome comprehensively. In general proteomics, high-resolution two-dimensional gel electrophoresis is primarily performed to isolate proteins as discrete spots followed by tryptic digestion and mass spectrometry for identification of protein and determination of modification. Thus we are performing proteomics to investigate proteins responsible for cellular aging and age-related diseases.

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